Dynamic-Clamp: From Principles to Applications / Edition 1

by Alain Destexhe, Thierry Bal

Overview

Dynamic-clamp is a fascinating electrophysiology technique that consists of merging living neurons with computational models. The dynamic-clamp (also called "conductance injection") allows experimentalists and theoreticians to challenge neurons (or any other type of cell) with complex conductance stimuli generated by a computer.

The technique can be implemented from neural simulation environments and a variety of custom-made or commercial systems. The real time interaction between the computer and cell also enables the design of recording paradigms with unprecedented accuracy via a computational model of the electrode. Dynamic-Clamp: From Principles to Applications contains contributions from leading researchers in the field, who investigate these paradigms at the cellular or network level, in vivo and in vitro, and in different brain regions and cardiac cells. Topics discussed include the addition of artificially-generated synaptic activity to neurons; adding, amplifying or neutralizing voltage-dependent conductances; creating hybrid networks with real and artificial cells; attaching simulated dendritic tree structures to the living cell; and connecting different neurons.

This book will be of interest to experimental biophysicists, neurophysiologists, and cardiac physiologists, as well as theoreticians, engineers, and computational neuroscientists. Graduate and undergraduate students will also find up to-date coverage of physiological problems and how they are investigated.

Product Details

ISBN-13:	9781441927903
Publisher:	Springer New York
Publication date:	11/05/2010
Series:	Springer Series in Computational Neuroscience , #1
Edition description:	Softcover reprint of hardcover 1st ed. 2009
Pages:	429
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

About the Author

Dr. Alain Destexhe and Dr.Thierry Bal, are Research Directors at the Centre National de la Recherche Scientifique (CNRS), a governmental research institution in France. Their laboratories are located in the CNRS campus of Gif-sur-Yvette in the research unit (Unité de Neurosciences Intégratives et Computationnelles, UNIC). Gif-sur-Yvette is solely devoted to research and provides an ideal environment for close interaction between theory and experiments, as exemplified by the numerous publications on dynamic-clamp experiments co-authored by Bal and Destexhe. Each editor holds Ph.D.s in both biophysics and neurobiology, respectively. Dr. Destexhe is also Chief Editor of the Journal of Computational Neuroscience.

Table of Contents

Associating Living Cells and Computational Models: an Introduction to Dynamic Clamp Principles and its Applications Zuzanna Piwkowska Alain Destexhe Thierry Bal 1

Dendritic Dynamic Clamp - A Tool to Study Single Neuron Computation Stephen R. Williams 31

Synaptic Conductances and Spike Generation in Cortical Cells Hugh P.C. Robinson 49

Simulating In Vivo Background Activity in a Slice with the Dynamic Clamp Frances Chance Larry F. Abbott 73

Impact of Background Synaptic Activity on Neuronal Response Properties Revealed by Stepwise Replication of In Vivo-Like Conditions In Vitro Steven A. Prescott Yves De Koninck 89

Testing Methods for Synaptic Conductance Analysis Using Controlled Conductance Injection with Dynamic Clamp Zuzanna Piwkowska Martin Pospischil Michelle Rudolph-Lilith Thierry Bald Alain Destexhe 115

In Vivo Dynamic-Clamp Manipulation of Extrinsic and Intrinsic Conductances: Functional Roles of Shunting Inhibition and IBK in Rat and Cat Cortex Lyle J. Graham Adrien Schramm 141

Functions of the Persistent Na+ Current in Cortical Neurons Revealed by Dynamic Clamp J.F. Storm K. Vervaeke H. Hu L.J. Graham 165

Using "Hard" Real-Time Dynamic Clamp to Study Cellular and Network Mechanisms of Synchronization in the Hippocampal Formation John A. White Fernando R. Fernandez Michael N. Economo Tilman J. Kispersky 199

Unraveling the Dynamics of Deep Cerebellar Nucleus Neurons with the Application of Artificial Conductances Dieter Jaeger Risa Lin 217

Intrinsic and Network Contributions to Reverberatory Activity: Reactive Clamp and Modeling Studies Jean-Marc Fellous Terrence J. Sejnowski Zaneta Navratilova 237

Dynamic-Clamp-Constructed Hybrid Circuits for theStudy of Synchronization Phenomena in Networks of Bursting Neurons Carmen C Canavier Fred H. Sieling Astrid A. Prinz 261

Using the Dynamic Clamp to Explore the Relationship Between Intrinsic Activity and Network Dynamics Anne-Elise Tobin Rachel Grashow Lamont S. Tang Stefan R. Pulver Eve Marder 275

Re-Creating In Vivo-Like Activity and Investigating the Signal Transfer Capabilities of Neurons: Dynamic-Clamp Applications Using Real-Time Neuron Gerard Sadoc Gwendal LeMasson Bruno Foutry Yann Le Franc Zuzanna Piwkowska Alain Destexhe Thierry Bal 287

Using the Dynamic Clamp to Dissect the Properties and Mechanisms of Intrinsic Thalamic Oscillations Stuart W. Hughes Magor Löet;rincz David W. Cope Vincenzo Crunelli 321

Dynamic Clamp with High-Resistance Electrodes Using Active Electrode Compensation In Vitro and In Vivo Romain Brette Zuzanna Piwkowska Cyril Monier José Francisco Gómez González Yves Frégnac Thierry Bal Alain Destexhe 347

Key Factors for Improving Dynamic-Clamp Performance Robert Butera Risa Lin 383

Development of a Genetically Engineered Cardiac Pacemaker: Insights from Dynamic Action Potential Clamp Experiments Arie O. Verkerk Jan G. Zegers Antoni C.G. van Ginneken Ronald Wilders 399

Index 417