Lipid Metabolism in Signaling Systems

by John N. Fain (Editor)

Overview

Many of the phospholipases and even the receptor for inositol 1,4,5-trisphosphate have recently been cloned and sequenced and play an important role in neurotransmission. Methods developed in recent years for the study of the enzymes and intermediates involved in the regulation of neuronal signal transduction involving phospholipid turnover are presented in this volume.

• Techniques for examining the role of phosphoinositides in signal transduction
• Techniques related to protein kinase C
• Analysis of inositol phosphates
• Techniques to analyze phospholipid turnover in the brain

Product Details

ISBN-13:	9781483288321
Publisher:	Elsevier Science
Publication date:	10/22/2013
Series:	Methods in Neurosciences , #18
Sold by:	Barnes & Noble
Format:	eBook
Pages:	357
File size:	7 MB

About the Author

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Table of Contents

Phospholipases Involved in Signaling Systems:
M.C. Hoener and U. Brodbeck, Phosphatidylinositol Glycan-Anchor-Specific Phospholipase D from Mammalian Brain.
M. Danin, V. Chalifa, H. M246;hn, U.-S. Schmidt, and M. Liscovitch, Rat Brain Membrane-Bound Phospholipase D.
M.M. Billah, J.C. Anthes, and P. Wang, Soluble Phospholipase D.
S. Llahi and J.N. Fain, Phosphatidylethanol Formation as an Index of Phospholipase D Activity in Rat Brain Cortex Slices.
E. Claro, F. Picatoste, and J.N. Fain, Antonist Stimulation of Phosphoinositide Breakdown in Brain Membranes.
M.A. Wallace, Regulation of Phosphoinositide Metabolism in Membranes Prepared from Human Brain Cortex.
D.-Y. Jhon, D. Park, and S.G. Rhee, Purification of Phospholipase C-b3 from Rat Brain.
C.-W. Lee, K.-H. Lee, and S.G. Rhee, Purification of Phospholipase C-b4 and Phospholipase C-d2 from Bovine Retinas.
A.V. Smrcka and P.C. Sternweis, Purification of aq/11 from Brain.
Other Enzymes Involved in Phopholipid Metabolism:
A. Ghalayini and J. Eichberg, Purification of Phosphatidylinositol Synthase from Brain.
A. Yamakawa and T. Takenawa, Phosphatidylinositol 4-Kinase from Bovine Brain.
S.P. Soltoff, D.R. Kaplan, and L.C. Cantley, Phosphatidylinositol 3-Kinase.
R. Parthasarathy, L. Parthasarathy, and R.E. Vadnal, Identification of Phosphatidylinositol Trisphosphate in Rat Brain.
Protein Kinase C:
F.L. Huang, K.-P. Huang, F.-S. Sheu, and K.-I. Osada, Preparation of Protein Kinase C Isozymes and Substrates from Rat Brain.
A.F.G. Quest, E.S.G. Bardes, J. Bloomenthal, R.A. Borhardt, and R.M. Bell, Protein Kinase C, a Zinc Metalloprotein: Quantitation of Zinc by Atomic Absorption Spectrometry.
S. Stabel, M. Liyanage, and D. Frith, Expression of Protein Kinase C Isozymes in Insect Cells and Isolation of Recombinant Proteins.
S. Jaken and K. Leach, Immunochemical Localization of Protein Kinase C and Phosphoinositide-Specific Phospholipase C.
C. Lee and A.K. Hajra, Quantitative Analysis of Molecular Species of Diacylglycerol in Biological Samples.
Measurement of Inositol Phosphates and Enzymes Regulating Inositol Phosphate Metabolism:
L.J. Rubin, F.-F. Hsu, and W.R. Sherman, Measurement of Inositol Trisphosphate by Gas Chromatography/MassSpectrometry: Femtomole Sensitivity Provided by Negative-Ion Chemical Ionization Mass Spectrometry in Submilligram Quantities of Tissue.
N. Sasakawa, T. Nakaki, and R. Kato, Characterization of Inositol Phosphates by High-Performance Liquid Chromatography.
R.A.J. Challiss and S.R. Nahorski, Measurement of Inositol 1,4,5-Trisphosphate, Inositol 1,3,4,5-Tetrakisphosphate, and Phosphatidylinositol 4,5-Bisphosphate in the Brain.
Inositol Phosphate Receptors and Their Regulation:
G.A. Mignery and T.C. Südhof, Molecular Analysis of Inositol 1,4,5-Trisphosphate Receptors.
R.J.H. Wojcikiewicz and S.R. Nahorski, Inositol 1,4,5-Trisphosphate Receptor Down-Regulation.
G. Reiser, High-Affinity Inositol 1,3,4,5-Tetrakisphosphate Receptor from Cerebellum.
M. Hirata and T. Kanematsu, Inositol 1,4,5-Trisphosphate-Binding Proteins in Rat Brain Cytosol.
C. Erneux, K. Takazawa, and B. Verjans, Inositol 1,4,5-Trisphosphate Phosphatase and Kinase from Brain.
A. Höer and E. Oberdisse, Synthesis of ³²P-Labeled Phosphoinositides and Inositol Phosphates: Characterization and Purification of Inositol 1,3,4,5-Tetrakisphosphate-3-phosphate from Brain.
L.E. Hokin and J.F. Dixon, Species Differences in the Response of Second Messenger Inositol 1,4,5-Trisphosphate to Lithium.
Index.