Thin Layer Chromatography in Phytochemistry / Edition 1

by Monika Waksmundzka-Hajnos, Joseph Sherma, Teresa Kowalska

Overview

Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants.

Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology.

Elucidates the role of plant materials in the pharmaceutical industry...

Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection.

Demonstrates practical methods that can be applied to a wide range of disciplines...

From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.

Product Details

ISBN-13:	9781420046779
Publisher:	CRC Press
Publication date:	03/04/2008
Series:	Chromatographic Science Series
Pages:	896
Product dimensions:	6.12(w) x 9.25(h) x (d)

Table of Contents

PART I
Overview of the Field of TLC in Phytochemistry and the Structure of the Book; M. Waksmundzka-Hajnos, J. Sherma, and T. Kowalska
Plant Materials in Modern Pharmacy and Methods of Their Investigations; K. Skalicka-Wozniak, J. Widelski, and K. Głowniak
Medicines and Dietary Supplements Produced from Plants; A. Ankli, V. Widmer, and E. Reich
Primary and Secondary Metabolites and Their Biological Activity; I. Chinou
Plant Chemosystematics; C. Zidorn
Sorbents and Precoated Layers for the Analysis and Isolation of Primary and Secondary Metabolites; J. Sherma
Chambers, Sample Application, and Chromatogram Development; T.H. Dzido and T. Tuzimski
Derivatization, Detection (Quantification), and Identification of Compounds Online; B. Spangenberg
Biodetection and Determination of Biological Activity of Natural Compounds; E. Tyihák, Á. M. Móricz, and P.G. Ott
Forced-Flow Planar Layer Liquid Chromatographic Techniques for the Separation and Isolation of Natural Substances; E. Mincsovics
PART II
Primary Metabolites
TLC of Carbohydrates; G.L. Sassaki, L.M. de Souza, Thales R. Cipriani, and M. Iacomini
TLC of Lipids; S. Momchilova and B. Nikolova-Damyanova
Amino Acids; R. Bhushan
Secondary Metabolites—Shickimic Acid Derivatives
Sample Preparation and TLC Analysis of Phenolic Acids; M. Wójciak-Kosior and A. Oniszczuk
Application of TLC in the Isolation and Analysis of Coumarins; M. Waksmundzka-Hajnos and M.A. Hawrył
Application of TLC in the Isolation and Analysis of Flavonoids; M. Medić-Šarić, I. Jasprica, A. Mornar, and Z. Maleš
TLC of Lignans; L. Opletal and H. Sovová
Secondary Metabolites—Isoprenoids
TLC of Mono- and Sesquiterpenes; A. Koch, S. Basar, and R. Richter
TLC of Diterpenes; M. Hajnos
TLC of Triterpenes (Including Saponins); W. Oleszek, I. Kapusta, and A. Stochmal
TLC of Carotenoids; G. Britton
TLC of Sterols, Steroids, and Related Triterpenoids; L. Dinan, J. Harmatha, and R. Lafont
TLC of Iridoids; G. Zgórka
Secondary Metabolites—Amino Acid Derivatives
TLC of Indole Alkaloids; P.J. Houghton
TLC of Isoquinoline Alkaloids; M. Waksmundzka-Hajnos and A. Petruczynik
TLC of Tropane Alkaloids; T. Mroczek
TLC of Alkaloids from the Other Biosynthetic Groups; J. Flieger
Secondary Metabolites—Compounds Derived from Acetogenine (Acetylocoenzyme A)
Polyacetylenes: Distribution in Higher Plants, Pharmacological Effects, and Analysis; Lars P. Christensen and H.B. Jakobsen
Quinone Derivatives in Plant Extracts; G. Matysik, A. Skalska-Kaminska, and A. Matysik-Wozniak
Index